RGN constructs delivered by bacteriophage particles (ΦRGN) exhibit efficient and specific antimicrobial effects against strains harboring plasmid or chromosomal target sequences.

RGN constructs delivered by bacteriophage particles (ΦRGN) exhibit efficient and specific antimicrobial effects against strains harboring plasmid or chromosomal target sequences.

Once delivered into a cell the RGN construct will attempt to locate and destroy the target gene. If the gene is absent (i) then the construct will exert no effect, whilst if the gene is chromosomal (ii) its destruction by RGN will result in cell death. If the gene is episomal (iii), so existing on a plasmid, the cell will either die or just experience loss of the plasmid, depending on whether or not a biochemical mechanism termed the “toxin-antitoxin system” is present.